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rad21 tev (Santa Cruz Biotechnology)

Name: rad21 tev
Brand: Santa Cruz Biotechnology
Rating: 96 (11174 reviews)

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a PCR analysis of <t>Rad21</t> alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b Schematic of ERt2-TEV-dependent RAD21-TEV degradation. c Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 7, h = hours). d Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24 h ( n = 3). A total of 463 genes were down- and 287 genes were upregulated (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Differentially expressed (DE) genes are shown in red. e Bar graph of individual GO terms and broad categories ( P < 1 × 10 −4 , Wallenius approximation) in RNA-seq of RAD21-TEV neurons treated as in ( d ). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. One-sided Fisher’s exact test was applied for the odds ratio and P value. All comparisons P < 2.22e-16. g Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV ( DE differentially expressed, R = Pearson correlation coefficient; P < 2.2e-16, two-sided F test). h 4 C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20th to 80th percentiles and the black line within shows mean values for 40 kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2 kb to 5 kb for n = 4 independent biological replicates. All replicates showed comparable results and were merged to generate this figure. i Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Rad21 Tev, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rad21 tev/product/Santa Cruz Biotechnology
Average 96 stars, based on 11174 article reviews

rad21 tev - by Bioz Stars, 2026-02

96/100 stars

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1) Product Images from "Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin"

Article Title: Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin

Journal: Nature Communications

doi: 10.1038/s41467-021-23141-9

a PCR analysis of Rad21 alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b Schematic of ERt2-TEV-dependent RAD21-TEV degradation. c Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 7, h = hours). d Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24 h ( n = 3). A total of 463 genes were down- and 287 genes were upregulated (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Differentially expressed (DE) genes are shown in red. e Bar graph of individual GO terms and broad categories ( P < 1 × 10 −4 , Wallenius approximation) in RNA-seq of RAD21-TEV neurons treated as in ( d ). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. One-sided Fisher’s exact test was applied for the odds ratio and P value. All comparisons P < 2.22e-16. g Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV ( DE differentially expressed, R = Pearson correlation coefficient; P < 2.2e-16, two-sided F test). h 4 C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20th to 80th percentiles and the black line within shows mean values for 40 kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2 kb to 5 kb for n = 4 independent biological replicates. All replicates showed comparable results and were merged to generate this figure. i Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Figure Legend Snippet: a PCR analysis of Rad21 alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b Schematic of ERt2-TEV-dependent RAD21-TEV degradation. c Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 7, h = hours). d Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24 h ( n = 3). A total of 463 genes were down- and 287 genes were upregulated (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Differentially expressed (DE) genes are shown in red. e Bar graph of individual GO terms and broad categories ( P < 1 × 10 −4 , Wallenius approximation) in RNA-seq of RAD21-TEV neurons treated as in ( d ). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. One-sided Fisher’s exact test was applied for the odds ratio and P value. All comparisons P < 2.22e-16. g Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV ( DE differentially expressed, R = Pearson correlation coefficient; P < 2.2e-16, two-sided F test). h 4 C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20th to 80th percentiles and the black line within shows mean values for 40 kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2 kb to 5 kb for n = 4 independent biological replicates. All replicates showed comparable results and were merged to generate this figure. i Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Techniques Used: Western Blot, Expressing, Gene Expression, RNA Sequencing, Transduction, Hi-C, Control, Binding Assay

a GSEA of RAD21-TEV downregulated genes in CdLS NeuN-positive RNAseq (left). GSEA of CdLS NeuN-positive downregulated genes in RAD21-TEV (right). NES normalized enrichment score, FDR false discovery rate. b Heatmap of log 2 fold-change for selected genes from CdLS NeuN-positive and RAD21-TEV samples separated into broad functional categories. Genes shown were significantly deregulated in CdLS and their mouse orthologs deregulated in at least one RAD21-TEV (ERt2-TEV or NLS-TEV) system. Genes highlighted in red were identified in the SFARI database, those in blue were deregulated in ASD, genes highlighted in orange fulfil both criteria. c Comparison of RAD21 (cohesin) binding (top), CTCF binding (center) and enhancer proximity (bottom, see methods) of genes deregulated (adj P < 0.05) in NeuN-positive CdLS (left) compared to genes deregulated in both NeuN-positive CdLS and RAD21-TEV (right). Up- and downregulation indicates the direction of deregulation in CdLS NeuN-positive RNAseq. One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Figure Legend Snippet: a GSEA of RAD21-TEV downregulated genes in CdLS NeuN-positive RNAseq (left). GSEA of CdLS NeuN-positive downregulated genes in RAD21-TEV (right). NES normalized enrichment score, FDR false discovery rate. b Heatmap of log 2 fold-change for selected genes from CdLS NeuN-positive and RAD21-TEV samples separated into broad functional categories. Genes shown were significantly deregulated in CdLS and their mouse orthologs deregulated in at least one RAD21-TEV (ERt2-TEV or NLS-TEV) system. Genes highlighted in red were identified in the SFARI database, those in blue were deregulated in ASD, genes highlighted in orange fulfil both criteria. c Comparison of RAD21 (cohesin) binding (top), CTCF binding (center) and enhancer proximity (bottom, see methods) of genes deregulated (adj P < 0.05) in NeuN-positive CdLS (left) compared to genes deregulated in both NeuN-positive CdLS and RAD21-TEV (right). Up- and downregulation indicates the direction of deregulation in CdLS NeuN-positive RNAseq. One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Techniques Used: Functional Assay, Comparison, Binding Assay

a Schematic of NLS-TEV mediated RAD21-TEV degradation and rescue. b Western blot of RAD21-TEV protein expression over a time course of Dox treatment (6 h, 100 ng/ml). Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 4). c Volcano plots of gene expression log 2 fold-change versus adjusted P value in RAD21-TEV + Dox treated neurons before and after rescue of RAD21-TEV expression ( n = 3). Left: 570 genes were down- and 150 genes were upregulated following RAD21-TEV depletion (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Right: 43 genes were down- and 44 genes were upregulated after rescue of RAD21-TEV expression (adj P < 0.05, shown in red and orange, Wald Test, Benjamini-Hochberg adjusted). Genes in red are significantly deregulated in both RAD21-TEV depletion and rescue, genes in orange are significantly deregulated only after rescue ( DE differentially expressed). d Log 2 fold-change of significantly deregulated genes (adj P < 0.05) following RAD21-TEV depletion (left) and after rescue (right). e The number of significantly deregulated genes (adj P < 0.05) after RAD21-TEV depletion (in green) and after rescue of RAD21-TEV expression (in orange). DE differentially expressed. f Heatmap of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their maturation trajectory in control (left) and +Dox treated samples (right). g Barcode plot of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their enrichment for directionality in maturation. Top: significantly de novo upregulated genes following rescue are enriched for genes upregulated during neuronal maturation. Bottom: de novo downregulated genes after cohesin rescue are enriched for genes that are downregulated during neuronal maturation.

Figure Legend Snippet: a Schematic of NLS-TEV mediated RAD21-TEV degradation and rescue. b Western blot of RAD21-TEV protein expression over a time course of Dox treatment (6 h, 100 ng/ml). Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 4). c Volcano plots of gene expression log 2 fold-change versus adjusted P value in RAD21-TEV + Dox treated neurons before and after rescue of RAD21-TEV expression ( n = 3). Left: 570 genes were down- and 150 genes were upregulated following RAD21-TEV depletion (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Right: 43 genes were down- and 44 genes were upregulated after rescue of RAD21-TEV expression (adj P < 0.05, shown in red and orange, Wald Test, Benjamini-Hochberg adjusted). Genes in red are significantly deregulated in both RAD21-TEV depletion and rescue, genes in orange are significantly deregulated only after rescue ( DE differentially expressed). d Log 2 fold-change of significantly deregulated genes (adj P < 0.05) following RAD21-TEV depletion (left) and after rescue (right). e The number of significantly deregulated genes (adj P < 0.05) after RAD21-TEV depletion (in green) and after rescue of RAD21-TEV expression (in orange). DE differentially expressed. f Heatmap of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their maturation trajectory in control (left) and +Dox treated samples (right). g Barcode plot of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their enrichment for directionality in maturation. Top: significantly de novo upregulated genes following rescue are enriched for genes upregulated during neuronal maturation. Bottom: de novo downregulated genes after cohesin rescue are enriched for genes that are downregulated during neuronal maturation.

Techniques Used: Western Blot, Expressing, Gene Expression, Control

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Journal: Nature Communications

Article Title: Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin

doi: 10.1038/s41467-021-23141-9

Figure Lengend Snippet: a PCR analysis of Rad21 alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b Schematic of ERt2-TEV-dependent RAD21-TEV degradation. c Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 7, h = hours). d Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24 h ( n = 3). A total of 463 genes were down- and 287 genes were upregulated (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Differentially expressed (DE) genes are shown in red. e Bar graph of individual GO terms and broad categories ( P < 1 × 10 −4 , Wallenius approximation) in RNA-seq of RAD21-TEV neurons treated as in ( d ). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. One-sided Fisher’s exact test was applied for the odds ratio and P value. All comparisons P < 2.22e-16. g Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV ( DE differentially expressed, R = Pearson correlation coefficient; P < 2.2e-16, two-sided F test). h 4 C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20th to 80th percentiles and the black line within shows mean values for 40 kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2 kb to 5 kb for n = 4 independent biological replicates. All replicates showed comparable results and were merged to generate this figure. i Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Resolved gels were blotted on to polyvinylidene fluoride transfer membrane, followed by 1 h incubation in fluorescent blocker (Millipore), and then incubated in primary antibody diluted to the appropriate amount in fluorescent blocker (Millipore) overnight at 4 o C. Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216), mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), rabbit polyclonal to NIPBL (1:1000; A301-779A, Bethyl Laboratories) mouse monoclonal anti-Myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40), mouse monoclonal to NLGN1 (1:100, Santa Cruz biotechnology sc-365110) and goat polyclonal antibody to SYN1(1:2500, Synaptic Systems, 106103).

Techniques: Western Blot, Expressing, Gene Expression, RNA Sequencing, Transduction, Hi-C, Control, Binding Assay

Journal: Nature Communications

Article Title: Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin

doi: 10.1038/s41467-021-23141-9

Figure Lengend Snippet: a GSEA of RAD21-TEV downregulated genes in CdLS NeuN-positive RNAseq (left). GSEA of CdLS NeuN-positive downregulated genes in RAD21-TEV (right). NES normalized enrichment score, FDR false discovery rate. b Heatmap of log 2 fold-change for selected genes from CdLS NeuN-positive and RAD21-TEV samples separated into broad functional categories. Genes shown were significantly deregulated in CdLS and their mouse orthologs deregulated in at least one RAD21-TEV (ERt2-TEV or NLS-TEV) system. Genes highlighted in red were identified in the SFARI database, those in blue were deregulated in ASD, genes highlighted in orange fulfil both criteria. c Comparison of RAD21 (cohesin) binding (top), CTCF binding (center) and enhancer proximity (bottom, see methods) of genes deregulated (adj P < 0.05) in NeuN-positive CdLS (left) compared to genes deregulated in both NeuN-positive CdLS and RAD21-TEV (right). Up- and downregulation indicates the direction of deregulation in CdLS NeuN-positive RNAseq. One sided Fisher’s exact test was applied for the odds ratio and P value. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Techniques: Functional Assay, Comparison, Binding Assay

Journal: Nature Communications

Article Title: Neuronal genes deregulated in Cornelia de Lange Syndrome respond to removal and re-expression of cohesin

doi: 10.1038/s41467-021-23141-9

Figure Lengend Snippet: a Schematic of NLS-TEV mediated RAD21-TEV degradation and rescue. b Western blot of RAD21-TEV protein expression over a time course of Dox treatment (6 h, 100 ng/ml). Bar plot of RAD21-TEV protein expression normalized to LAMIN B ( n = 4). c Volcano plots of gene expression log 2 fold-change versus adjusted P value in RAD21-TEV + Dox treated neurons before and after rescue of RAD21-TEV expression ( n = 3). Left: 570 genes were down- and 150 genes were upregulated following RAD21-TEV depletion (adj P < 0.05, Wald Test, Benjamini-Hochberg adjusted). Right: 43 genes were down- and 44 genes were upregulated after rescue of RAD21-TEV expression (adj P < 0.05, shown in red and orange, Wald Test, Benjamini-Hochberg adjusted). Genes in red are significantly deregulated in both RAD21-TEV depletion and rescue, genes in orange are significantly deregulated only after rescue ( DE differentially expressed). d Log 2 fold-change of significantly deregulated genes (adj P < 0.05) following RAD21-TEV depletion (left) and after rescue (right). e The number of significantly deregulated genes (adj P < 0.05) after RAD21-TEV depletion (in green) and after rescue of RAD21-TEV expression (in orange). DE differentially expressed. f Heatmap of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their maturation trajectory in control (left) and +Dox treated samples (right). g Barcode plot of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their enrichment for directionality in maturation. Top: significantly de novo upregulated genes following rescue are enriched for genes upregulated during neuronal maturation. Bottom: de novo downregulated genes after cohesin rescue are enriched for genes that are downregulated during neuronal maturation.

Techniques: Western Blot, Expressing, Gene Expression, Control

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